Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

Comparison of protein patterns after two-dimensional gel electrophoresis from leaves of in vitro cultures and seedlings of Rubus chamaemorus L.

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Proteins from leaves of Rubus chamaemorus propagated in vitro were subjected to miniaturized 2-D electrophoresis.The 2-DE patterns of proteins showed qualitative differences between plants propagated in vitro mug washer and control seedlings.More proteins of a high molecular weight were observed in leaves of plants from in vitro culture.A two-dimensional map of proteins from leaves provides detailed data concerning both polymorphism and protein patterns cucharas reservoir of this species.This makes it possible to start constructing a protein map of R.

chamaemorus.The reasons for qualitative differences are discussed.